6DB9
Structural basis for promiscuous binding and activation of fluorogenic dyes by DIR2s RNA aptamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979200 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.780, 83.805, 109.926 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.640 - 2.025 |
R-factor | 0.2311 |
Rwork | 0.230 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zke |
RMSD bond length | 0.003 |
RMSD bond angle | 0.758 |
Phasing software | PHENIX (1.11.1_2575) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.810 | 2.080 |
High resolution limit [Å] | 2.025 | 2.025 |
Rmerge | 0.040 | 1.566 |
Rmeas | 0.044 | |
Rpim | 0.019 | 0.811 |
Number of reflections | 50597 | 3602 |
<I/σ(I)> | 20.1 | 0.9 |
Completeness [%] | 99.3 | 96.9 |
Redundancy | 5.1 | 4.9 |
CC(1/2) | 0.995 | 0.380 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15 % v/v 2-Propanol, 50 mM MES pH 6.0 20 mM MgCl2 |