6DAT

ETS1 in complex with synthetic SRR mimic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 21 2
Unit cell lengths	89.111, 89.111, 215.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.188 - 2.350
R-factor	0.234759949156
Rwork	0.233
R-free	0.26801
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6da1
RMSD bond length	0.003
RMSD bond angle	0.506
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.190	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.133	1.898
Rpim	0.038	0.550
Number of reflections	37109	8714
<I/σ(I)>	12.8
Completeness [%]	99.8
Redundancy	13
CC(1/2)	0.999	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	MES, PEG 3350, lithium sulfate