6DAN
PhdJ WT 2 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.406, 136.946, 88.264 |
| Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.047 |
| R-factor | 0.18 |
| Rwork | 0.176 |
| R-free | 0.22100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.798 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 72626 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 94.3 | |
| Redundancy | 3.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298.15 | 0.1 M MES at pH 7-7.5 and 22-25% in PEG 3350 |
