6D9G

X-ray Structure of the FAB Fragment of 15B8, a Murine Monoclonal Antibody Specific for the Human Serotonin Transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 2 21 21
Unit cell lengths	82.870, 85.490, 141.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 2.300
R-factor	0.1965
Rwork	0.195
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i66
RMSD bond length	0.002
RMSD bond angle	0.595
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_2597)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.930	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.092	0.413
Number of reflections	45074	4199
<I/σ(I)>	17.88	2.68
Completeness [%]	99.1	94.2
Redundancy	10.8	4.6
CC(1/2)	0.999	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.2 M ammonium sulfate, 0.08 M sodium citrate pH 5.2