6D9F
Protein 60 with aldehyde deformylating oxidase activity from Kitasatospora setae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.630, 79.030, 124.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.760 - 2.030 |
| R-factor | 0.19957 |
| Rwork | 0.198 |
| R-free | 0.23395 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.588 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.760 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.059 | 0.362 |
| Number of reflections | 50138 | 3667 |
| <I/σ(I)> | 15.8 | 4.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.075 M TRIS hydrochloride pH 8.5, 1.5 M Ammonium sulfate, 25% v/v Glycerol |
