6D95
Ternary RsAgo Complex with Guide RNA Paired and Target DNA containing A8-A8' Non-Canonical Pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.197, 119.978, 118.692 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 37.440 - 1.850 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.25000 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.058 | 0.602 |
| Number of reflections | 159065 | |
| <I/σ(I)> | 12.8 | 1.8 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 3 | 2.8 |
| CC(1/2) | 0.989 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | from 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7 |
