6D95

Ternary RsAgo Complex with Guide RNA Paired and Target DNA containing A8-A8' Non-Canonical Pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	68.197, 119.978, 118.692
Unit cell angles	90.00, 95.51, 90.00

Refinement procedure

Resolution	37.440 - 1.850
R-factor	0.2117
Rwork	0.210
R-free	0.25000
RMSD bond length	0.011
RMSD bond angle	1.539
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.058	0.602
Number of reflections	159065
<I/σ(I)>	12.8	1.8
Completeness [%]	98.4	98.4
Redundancy	3	2.8
CC(1/2)	0.989	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	from 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7