6D8P
Ternary RsAgo Complex Containing Guide RNA Paired with Target DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.234, 119.216, 117.648 |
Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
Resolution | 42.336 - 2.100 |
R-factor | 0.1843 |
Rwork | 0.182 |
R-free | 0.23480 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 101956 | |
<I/σ(I)> | 27.1 | |
Completeness [%] | 93.5 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 40% MPD, 30 mM magnesium acetate, 50 mM sodium cacodylate pH 5.7 |