6D8L

Group I self-splicing intron P4-P6 domain mutant U131A (without isopropanol soaking)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979100
Spacegroup name	P 21 21 21
Unit cell lengths	75.480, 132.950, 147.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.197 - 3.140
R-factor	0.251022611814
Rwork	0.250
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gid
RMSD bond length	0.003
RMSD bond angle	0.732
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.200	3.220
High resolution limit [Å]	3.140	3.140
Number of reflections	25498
<I/σ(I)>	12.9
Completeness [%]	95.9
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	MPD, sodium cacodylate, cobalt hexamine