6D8B

The crystal structure of hemagglutinin from A/Hong Kong/125/2017 H7N9 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	202.642, 116.891, 119.628
Unit cell angles	90.00, 124.16, 90.00

Refinement procedure

Resolution	39.154 - 2.950
R-factor	0.2366
Rwork	0.234
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ln6
RMSD bond length	0.008
RMSD bond angle	1.334
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2733: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.950	2.950
Number of reflections	48207
<I/σ(I)>	18.39
Completeness [%]	98.9
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2M Magnesium Acetate, 20% PEG 3350