6D8B
The crystal structure of hemagglutinin from A/Hong Kong/125/2017 H7N9 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 202.642, 116.891, 119.628 |
Unit cell angles | 90.00, 124.16, 90.00 |
Refinement procedure
Resolution | 39.154 - 2.950 |
R-factor | 0.2366 |
Rwork | 0.234 |
R-free | 0.27870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ln6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.334 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 48207 | |
<I/σ(I)> | 18.39 | |
Completeness [%] | 98.9 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.2M Magnesium Acetate, 20% PEG 3350 |