6D89
Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.650, 199.191, 84.625 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 29.345 - 2.000 |
| R-factor | 0.1768 |
| Rwork | 0.176 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.849 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.345 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 163378 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 98.4 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Sodium Citrate: Citric Acid, pH 5.5, 20 % (w/v) PEG 3000 |
