6D85
Structure of the Bovine p85a BH domain E217K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-10-25 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.474, 91.647, 93.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.787 - 2.203 |
| R-factor | 0.2031 |
| Rwork | 0.202 |
| R-free | 0.22070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.624 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.790 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 37700 | 1851 |
| <I/σ(I)> | 36.9 | 4.6 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.5 M Li2SO4, 100 mM Na Cacodylate, pH 6.0 |
