6D82
Structure of the Bovine p85a BH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-10-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.364, 91.924, 93.274 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.755 - 2.407 |
| R-factor | 0.1919 |
| Rwork | 0.190 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.760 | 2.440 |
| High resolution limit [Å] | 2.407 | 2.407 |
| Rmerge | 0.070 | 0.453 |
| Number of reflections | 28798 | 1419 |
| <I/σ(I)> | 31.7 | 4.8 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.5 M Li2SO4, 100mM Na Cacodylate pH 6.0 |
