6D81

Structure of the Bovine p85a BH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-10-25
Detector	RAYONIX MX300HE
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	85.825, 91.737, 93.778
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.889 - 2.248
R-factor	0.1927
Rwork	0.192
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pbw
RMSD bond length	0.003
RMSD bond angle	0.705
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	2.309
High resolution limit [Å]	2.248	2.248
Rmerge	0.094	0.638
Number of reflections	35631	2519
<I/σ(I)>	24.2	3
Completeness [%]	99.2	98.6
Redundancy	7.4	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.5 M Li2SO4, pH 6.0, 100mM Na Cacodylate