6D7F
Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 252.177, 252.177, 107.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.296 - 2.400 |
| R-factor | 0.1749 |
| Rwork | 0.174 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.909 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.111 | 0.511 |
| Number of reflections | 152030 | |
| <I/σ(I)> | 23.4 | 7 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Potassium Sulfate, 20% PEG 3350, 10mM Thiophenyl-beta-D-glucuronide |
