6D7A
Structure of T. gondii PLP1 beta-rich domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-25 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.980, 50.850, 105.340 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 24.883 - 1.130 |
R-factor | 0.1418 |
Rwork | 0.141 |
R-free | 0.16370 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.044 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.883 | 1.170 |
High resolution limit [Å] | 1.130 | 1.130 |
Rmerge | 0.077 | |
Rmeas | 0.084 | |
Rpim | 0.034 | |
Number of reflections | 191300 | |
<I/σ(I)> | 7.99 | |
Completeness [%] | 94.9 | |
Redundancy | 4.6 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 100 mM sodium cacodylate, 0.8M sodium formate, 15% PEG 4000 |