6D79
Structure of CysZ, a sulfate permease from Pseudomonas Fragi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97890 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.100, 57.020, 96.140 |
| Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
| Resolution | 39.599 - 3.501 |
| R-factor | 0.2964 |
| Rwork | 0.294 |
| R-free | 0.34370 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.039 | 0.388 |
| Number of reflections | 20742 | |
| <I/σ(I)> | 12.3 | 3.7 |
| Completeness [%] | 99.8 | |
| Redundancy | 27.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 277 | PEG400, MES pH 6.0 |
