6D6W

Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03319
Spacegroup name	C 1 2 1
Unit cell lengths	134.175, 133.920, 163.436
Unit cell angles	90.00, 100.31, 90.00

Refinement procedure

Resolution	29.793 - 1.800
R-factor	0.1806
Rwork	0.180
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.874
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.980	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	261936
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Sodium Citrate: Citrate Acid, pH 5.5, 20% PEG 3000, 20mM Glucuronic Acid