6D6E

Triclinic lysozyme (295 K) in the presence of 47% xylose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Agilent SuperNova
Temperature [K]	295
Detector technology	CCD
Collection date	2016-01-19
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	27.272, 32.236, 34.316
Unit cell angles	88.22, 71.18, 68.69

Refinement procedure

Resolution	13.359 - 2.000
R-factor	0.1382
Rwork	0.136
R-free	0.18690
RMSD bond length	0.002
RMSD bond angle	0.548
Data scaling software	SCALA
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.670	2.110
High resolution limit [Å]	2.000	2.000
Rmeas	0.071	0.241
Number of reflections	6847
<I/σ(I)>	14.5	5.2
Completeness [%]	98.9
Redundancy	3
CC(1/2)	0.997	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein: 10 mg/mL Well: 0.3 M NaNO3