6D69

Crystal Structure of the NHL Repeat Region of D. melanogaster Thin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 65
Unit cell lengths	132.727, 132.727, 49.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.445 - 2.601
R-factor	0.1894
Rwork	0.186
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q7f
RMSD bond length	0.014
RMSD bond angle	1.502
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.128	0.052	0.680
Number of reflections	15485	1633	1404
<I/σ(I)>	14.36
Completeness [%]	99.0	99.5	91.9
Redundancy	7.2	7.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.1 M HEPES (pH 7.8) 0.2 M sodium chloride 25% (w/v) peg-3350