6D57
Campylobacter jejuni ferric uptake regulator S1 metalated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.742, 84.363, 123.626 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.680 - 1.810 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.22900 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.680 | 1.900 |
High resolution limit [Å] | 1.810 | 1.810 |
Number of reflections | 34641 | |
<I/σ(I)> | 5.3 | |
Completeness [%] | 100.0 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Bis-Tris, pH 5.5, 0.25 M magnesium formate, 25% PEG3350 |