6D50

Bacteroides uniforms beta-glucuronidase 2 bound to D-glucaro-1,5-lactone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	74.489, 141.880, 180.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.476 - 2.500
R-factor	0.168
Rwork	0.166
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uj6
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.480	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	446802
<I/σ(I)>	15.9
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M Potassium Chloride, 20% PEG 3350, 10mM Saccharolactone