6D4M

Crystal Structure of a Fc Fragment of Rhesus macaque (Macaca mulatta) IgG3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9746
Spacegroup name	P 32
Unit cell lengths	65.866, 65.866, 89.115
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.114 - 3.470
R-factor	0.2526
Rwork	0.249
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.006
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.500
High resolution limit [Å]	3.450	3.450
Rmerge	0.242	0.802
Rpim	0.172	0.570
Number of reflections	5321
<I/σ(I)>	7.4	1.3
Completeness [%]	94.9	98
Redundancy	2.6	2.6
CC(1/2)	0.990	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	35% Tacsimate pH 7.0