6D4M
Crystal Structure of a Fc Fragment of Rhesus macaque (Macaca mulatta) IgG3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9746 |
Spacegroup name | P 32 |
Unit cell lengths | 65.866, 65.866, 89.115 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.114 - 3.470 |
R-factor | 0.2526 |
Rwork | 0.249 |
R-free | 0.27030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ave |
RMSD bond length | 0.006 |
RMSD bond angle | 1.031 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.242 | 0.802 |
Rpim | 0.172 | 0.570 |
Number of reflections | 5321 | |
<I/σ(I)> | 7.4 | 1.3 |
Completeness [%] | 94.9 | 98 |
Redundancy | 2.6 | 2.6 |
CC(1/2) | 0.990 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 35% Tacsimate pH 7.0 |