6D4I
Crystal Structure of a Fc Fragment of Rhesus macaque (Macaca mulatta) IgG2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 32 |
Unit cell lengths | 66.916, 66.916, 87.454 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.905 - 2.950 |
R-factor | 0.2095 |
Rwork | 0.207 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4haf |
RMSD bond length | 0.008 |
RMSD bond angle | 1.228 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.087 | 0.657 |
Rpim | 0.064 | 0.485 |
Number of reflections | 8919 | |
<I/σ(I)> | 13.6 | 1.3 |
Completeness [%] | 96.1 | 97.7 |
Redundancy | 2.6 | 2.7 |
CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 3 M sodium chloride 0.1 M sodium acetate pH 4.5 |