6D4G
N-GTPase domain of p190RhoGAP-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.690, 69.270, 154.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.032 - 2.800 |
R-factor | 0.252 |
Rwork | 0.250 |
R-free | 0.29010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c5h |
RMSD bond length | 0.008 |
RMSD bond angle | 0.663 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.032 | 42.032 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.179 | 0.085 | 3.529 |
Rmeas | 0.181 | 0.086 | 3.561 |
Total number of observations | 624368 | ||
Number of reflections | 12101 | 1307 | 1192 |
<I/σ(I)> | 20.41 | 48.85 | 1.87 |
Completeness [%] | 99.8 | 98.6 | 100 |
Redundancy | 51.596 | 45.59 | 54.501 |
CC(1/2) | 0.999 | 0.999 | 0.691 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 25% (w/v) PEG 8000, 100 mM Tris pH 8.5 |