6D4D
Crystal structure of the PTP epsilon D1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.055, 35.998, 115.023 |
Unit cell angles | 90.00, 115.73, 90.00 |
Refinement procedure
Resolution | 38.282 - 1.765 |
R-factor | 0.1797 |
Rwork | 0.178 |
R-free | 0.22230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jjd |
RMSD bond length | 0.006 |
RMSD bond angle | 0.810 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.082 | 0.542 |
Number of reflections | 56107 | |
<I/σ(I)> | 19.9 | |
Completeness [%] | 98.9 | |
Redundancy | 3.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris-HCl pH 8.5, 0.2 M sodium chloride, 22% (w/v) polyethylene glycol 3350 |