6D4A

Cell Surface Receptor with Bound Ligand at 1.75-A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	29.391, 68.339, 73.618
Unit cell angles	90.00, 97.45, 90.00

Refinement procedure

Resolution	34.170 - 1.751
R-factor	0.1779
Rwork	0.176
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d48
RMSD bond length	0.007
RMSD bond angle	1.001
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1-2575_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.170	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.072	0.676
Rmeas	0.083	0.785
Rpim	0.042	0.397
Number of reflections	28662	1477
<I/σ(I)>	13.4	2
Completeness [%]	98.4	94.2
Redundancy	3.8	3.7
CC(1/2)	0.998	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES pH 6.5 0.6 M NaCl 18% PEG 4000