6D48
Cell Surface Receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.801, 41.543, 107.498 |
Unit cell angles | 90.00, 96.55, 90.00 |
Refinement procedure
Resolution | 35.599 - 1.776 |
R-factor | 0.1774 |
Rwork | 0.176 |
R-free | 0.21220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nko |
RMSD bond length | 0.010 |
RMSD bond angle | 0.979 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1-2575_1496: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.480 | 1.810 |
High resolution limit [Å] | 1.776 | 1.776 |
Rmerge | 0.044 | 0.391 |
Rmeas | 0.052 | 0.464 |
Rpim | 0.028 | 0.246 |
Number of reflections | 51251 | 2673 |
<I/σ(I)> | 14.7 | 3.1 |
Completeness [%] | 98.5 | 90.3 |
Redundancy | 3.4 | 3.2 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG 4000 100 mM Tris-HCl pH 8.5 10% Glycerol |