6D47

Crystal structure of a DNA polymerase III subunit beta DnaN sliding clamp from Mycobacterium marinum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 32 2 1
Unit cell lengths	137.410, 137.410, 83.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.374 - 2.450
R-factor	0.1953
Rwork	0.193
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p16
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.374	48.374	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.066	0.031	0.560
Rmeas	0.072	0.035	0.611
Number of reflections	33494	412	2452
<I/σ(I)>	13.9	27.43	2.31
Completeness [%]	99.8	95.4	99.8
Redundancy	6.039	4.903	6.162
CC(1/2)	0.998	0.998	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287	MymaA.17987.a.B1.PW38239 at 22.54 mg/mL protein against Morpheus screen condition G6 = 10% PEG 8000, 20% EG, 0.02 M each carboxylic acid (sodium formate, ammonium acetate, trisodium citrate, sodium potassium L-tartrate, sodium oxamate), 0.1 M MOPS/HEPES pH 7.5, direct cryo, 290679g6, pcm7-8