6D46
Crystal structure of a DNA polymerase III subunit beta DnaN sliding clamp from Rickettsia typhi str. Wilmington
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 147.520, 43.050, 83.170 |
Unit cell angles | 90.00, 121.39, 90.00 |
Refinement procedure
Resolution | 41.533 - 2.000 |
R-factor | 0.1771 |
Rwork | 0.174 |
R-free | 0.21660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w7z |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.533 | 41.533 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.049 | 0.027 | 0.489 |
Rmeas | 0.056 | 0.032 | 0.559 |
Number of reflections | 30248 | 370 | 2239 |
<I/σ(I)> | 16 | 34.14 | 3.52 |
Completeness [%] | 98.7 | 96.1 | 98.4 |
Redundancy | 4.118 | 3.514 | 4.228 |
CC(1/2) | 0.999 | 0.998 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 287 | 24.49 mg/mL RityA.17987.a.B1.PW38354 against JCSG+ screen condition C6 = 0.1 M phosphate/citrate pH 4.2, 40% PEG 300, direct cryo, 295697c6, ffq4-2 |