6D41
Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03322 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.066, 130.156, 94.490 |
Unit cell angles | 90.00, 100.24, 90.00 |
Refinement procedure
Resolution | 29.934 - 1.900 |
R-factor | 0.1404 |
Rwork | 0.140 |
R-free | 0.16860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6d1n |
RMSD bond length | 0.007 |
RMSD bond angle | 0.855 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.934 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 114979 | |
<I/σ(I)> | 16.6 | |
Completeness [%] | 97.7 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 16% PEG 1000, Tris pH 8.5, 2mM Saccharolactone |