6D3P

Crystal structure of an exoribonuclease-resistant RNA from Sweet clover necrotic mosaic virus (SCNMV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-11-23
Detector	RDI CMOS_8M
Wavelength(s)	1.0972
Spacegroup name	P 61 2 2
Unit cell lengths	83.400, 83.400, 94.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.455 - 2.900
R-factor	0.2434
Rwork	0.241
R-free	0.25870
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.455	39.455	1.900
High resolution limit [Å]	1.850	8.280	1.850
Rmerge	0.592	0.054
Rmeas	0.612	0.054
Total number of observations	713044
Number of reflections	27657	357	542
<I/σ(I)>	7.14	81.55
Completeness [%]	88.6	98.3	23.7
Redundancy	25.782	43.667	1.696
CC(1/2)	0.884	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	drop: 1 uL 5 mg/mL RNA in 30 mM HEPES pH 7.5, 20 mM MgCl2, 100 mM KCl + 1 uL 50 mM sodium cacodylate trihydrate (pH 6.5), 0.2 M potassium chloride, 0.1 M magnesium acetate tetrahydrate, 14% w/v PEG 8000. Crystals were buffer-exchanged into freezing solution (mother liquor containing 20% ethylene glycol and 20 mM Iridium (III) hexammine) and flash-frozen in liquid nitrogen