6D3J

FT_T dioxygenase holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-03
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 62 2 2
Unit cell lengths	137.413, 137.413, 76.261
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	119.000 - 3.000
R-factor	0.1982
Rwork	0.195
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	2.221
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.000	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	8906
<I/σ(I)>	39.5
Completeness [%]	99.9	100
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	precipitant solution: 2.5 M NaCl, 0.1M KP04/NaP04-pH 6.2; protein solution: 10-20 mg/ml in 30 mM Tris-8, 0.2 M NaCl