6D3I
ftv7 dioxygenase with 2,4-D bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.927, 109.980, 116.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.701 - 3.196 |
| R-factor | 0.1858 |
| Rwork | 0.176 |
| R-free | 0.27300 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.513 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 20563 | 1037 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | -crystallization: Wizard 34 H7 = 30% MPD, 0.1M imidazole-HCl-pH 6.5, 0.2M ammonium sulfate, 10% PEG 3350 |
