6D2X

Crystal structure of the GH26 domain from PbGH26-GH5A endo-beta-mannanase/endo-beta-glucanase from Prevotella bryantii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-06-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	73.577, 73.577, 105.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.243 - 1.720
R-factor	0.1547
Rwork	0.153
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d2w
RMSD bond length	0.010
RMSD bond angle	0.992
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_3092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.082	1.142
Rpim	0.035	0.492
Number of reflections	35561
<I/σ(I)>	36.3	2.14
Completeness [%]	99.9	100
Redundancy	6.4	6.3
CC(1/2)		0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	2 M ammonium sulfate, 2% (w/v) PEG200, 15 mg/mL protein