6D2O
Beta Carbonic anhydrase in complex with 4-methylimidazole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-20 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 71.339, 77.511, 86.893 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.400 - 1.900 |
Rwork | 0.187 |
R-free | 0.22800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.400 | 2.100 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 17661 | |
<I/σ(I)> | 11.9 | |
Completeness [%] | 94.6 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50% PEG200; 20 mM Tris-HCl; pH 7.0 |