6D2K
Crystal structure of the FERM domain of mouse FARP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.418, 72.762, 94.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.380 - 1.550 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2he7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.380 |
| High resolution limit [Å] | 1.550 |
| Number of reflections | 37895 |
| <I/σ(I)> | 34.02 |
| Completeness [%] | 99.7 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Sodium acetate trihydrate, 12 % PEG 3350 (w/v) |
