6D2K
Crystal structure of the FERM domain of mouse FARP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.418, 72.762, 94.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.380 - 1.550 |
R-factor | 0.198 |
Rwork | 0.197 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2he7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.031 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.380 |
High resolution limit [Å] | 1.550 |
Number of reflections | 37895 |
<I/σ(I)> | 34.02 |
Completeness [%] | 99.7 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Sodium acetate trihydrate, 12 % PEG 3350 (w/v) |