6D25

Crystal structure of the GH51 arabinofuranosidase from Xanthomonas axonopodis pv. citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979500
Spacegroup name	P 1 21 1
Unit cell lengths	91.344, 163.231, 114.425
Unit cell angles	90.00, 107.57, 90.00

Refinement procedure

Resolution	48.740 - 1.910
R-factor	0.1488
Rwork	0.149
R-free	0.16670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vrq
RMSD bond length	0.012
RMSD bond angle	1.509
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.740	48.740	2.030
High resolution limit [Å]	1.910	5.700	1.910
Rmerge	0.095	0.043	0.514
Rmeas	0.113	0.051	0.639
Number of reflections	236094	18012	63391
<I/σ(I)>	9.74	25.62	2.06
Completeness [%]	95.1	97.7	80.3
Redundancy	3.429	3.614	2.81
CC(1/2)	0.996	0.997	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	17% (w/v) polyethylene glycol 3350 and 0.2 M ammonium chloride