6D22
Crystal structure of Tyrosine-protein kinase receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9724 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.120, 105.120, 203.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 2.460 |
| R-factor | 0.1784 |
| Rwork | 0.175 |
| R-free | 0.23610 |
| RMSD bond length | 0.036 |
| RMSD bond angle | 2.736 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | BUSTER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.906 | 19.906 | 2.590 |
| High resolution limit [Å] | 2.458 | 7.770 | 2.460 |
| Rmerge | 0.047 | 1.073 | |
| Rmeas | 0.161 | 0.056 | 1.391 |
| Rpim | 0.066 | 0.023 | 0.641 |
| Number of reflections | 15776 | 531 | 2028 |
| <I/σ(I)> | 9.2 | 10.6 | 0.7 |
| Completeness [%] | 98.1 | 94.1 | 88.4 |
| Redundancy | 5.7 | 5.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 0.1M tri-Sodium citrate + 20% PEG3K |
