6D1R

Structure of Staphylococcus aureus RNase P protein at 2.0 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 3 2 1
Unit cell lengths	72.159, 72.159, 58.958
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.246 - 1.995
R-factor	0.2161
Rwork	0.214
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.085	0.039	1.216
Rmeas	0.090	0.042	1.287
Rpim	0.030	0.014	0.418
Total number of observations	111835
Number of reflections	12313	1319	1214
<I/σ(I)>	7.3
Completeness [%]	100.0	99.8	100
Redundancy	9.1	8.6	9.4
CC(1/2)		0.998	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298.15	3.3M (NH4)2SO4, 0.15M potassium phosphate pH6.5