6D1Q

Crystal structure of E. coli RppH-DapF complex, monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979100
Spacegroup name	C 2 2 21
Unit cell lengths	161.440, 192.320, 50.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	123.650 - 2.150
R-factor	0.2135
Rwork	0.212
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d13
RMSD bond length	0.008
RMSD bond angle	1.051
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.650	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.049	1.557
Number of reflections	43324
<I/σ(I)>	15.2
Completeness [%]	99.1
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	30% (v/v) PEG400 and 0.1 M CHES, pH 9.2