6D1P

Apo structure of Bacteroides uniformis beta-glucuronidase 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03316
Spacegroup name	C 1 2 1
Unit cell lengths	209.721, 68.209, 167.300
Unit cell angles	90.00, 122.49, 90.00

Refinement procedure

Resolution	29.095 - 2.350
R-factor	0.1766
Rwork	0.175
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.480	2.390
High resolution limit [Å]	2.350	2.350
Number of reflections	83426
<I/σ(I)>	21
Completeness [%]	99.9
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	8% PEG 3350, 0.2 M Potassium Thiocyanate and 0.1M Hepes pH 8.0