6D11
Crystal structure of 1450 Fab in complex with circumsporozoite protein NANP5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.620, 135.058, 344.092 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.490 - 3.400 |
R-factor | 0.2563 |
Rwork | 0.254 |
R-free | 0.29930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.692 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.600 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.311 | 0.727 |
Rpim | 0.165 | 0.386 |
Number of reflections | 15950 | 2452 |
<I/σ(I)> | 4.5 | 1.3 |
Completeness [%] | 92.1 | 92.9 |
Redundancy | 3.9 | 4 |
CC(1/2) | 0.957 | 0.496 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 22.5% (w/v) PEG3350, 0.2M di-ammonium hydrogen citrate |