6D0R
Structure of a DNA retention-prone PCNA variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-25 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 3 |
Unit cell lengths | 121.716, 121.716, 121.716 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.490 - 2.859 |
R-factor | 0.243790221328 |
Rwork | 0.242 |
R-free | 0.27588 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.833 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 (v703x) |
Phasing software | PHASER |
Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.145 | 1.072 |
Rmeas | 0.149 | 1.102 |
Rpim | 0.033 | 0.256 |
Number of reflections | 14213 | 707 |
<I/σ(I)> | 25.99 | 2.35 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20.8 | 18.8 |
CC(1/2) | 0.999 | 0.858 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM sodium citrate pH 5.3 and 1.7 M (NH4)2SO4 |