6D0R

Structure of a DNA retention-prone PCNA variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-25
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54178
Spacegroup name	P 21 3
Unit cell lengths	121.716, 121.716, 121.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.490 - 2.859
R-factor	0.243790221328
Rwork	0.242
R-free	0.27588
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yhr
RMSD bond length	0.006
RMSD bond angle	0.833
Data reduction software	HKL-3000
Data scaling software	HKL-3000 (v703x)
Phasing software	PHASER
Refinement software	PHENIX (1.12-2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.145	1.072
Rmeas	0.149	1.102
Rpim	0.033	0.256
Number of reflections	14213	707
<I/σ(I)>	25.99	2.35
Completeness [%]	100.0	100
Redundancy	20.8	18.8
CC(1/2)	0.999	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM sodium citrate pH 5.3 and 1.7 M (NH4)2SO4