6D0Q
Structure of a DNA retention-prone PCNA variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-31 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 3 |
Unit cell lengths | 122.264, 122.264, 122.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.910 - 2.801 |
R-factor | 0.243303038576 |
Rwork | 0.242 |
R-free | 0.26913 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.950 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 (v703x) |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.093 | 1.154 |
Rmeas | 0.098 | 1.208 |
Rpim | 0.029 | 0.354 |
Number of reflections | 15284 | 757 |
<I/σ(I)> | 27.54 | 1.65 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.5 | 11.5 |
CC(1/2) | 0.996 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 50 mM sodium citrate pH 5.6, 2 M (NH4)2SO4 |