6D0M
Polymerase Eta post-insertion binary complex with cytarabine (AraC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-24 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.566, 98.566, 81.477 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.680 - 1.832 |
| R-factor | 0.1621 |
| Rwork | 0.160 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j9p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.074 | 0.612 |
| Number of reflections | 39467 | 1849 |
| <I/σ(I)> | 31.4 | 2.25 |
| Completeness [%] | 99.7 | 95.7 |
| Redundancy | 12 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MES buffer, PEG1500 |
