6D0L
Structure of human TIRR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.890, 46.360, 48.800 |
| Unit cell angles | 92.31, 108.07, 105.75 |
Refinement procedure
| Resolution | 38.000 - 1.970 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kvh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.041 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.084 | 0.338 |
| Rmeas | 0.098 | 0.396 |
| Number of reflections | 22070 | 1980 |
| <I/σ(I)> | 15.12 | 5.15 |
| Completeness [%] | 93.7 | 84.07 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.06 M citric acid, 0.04 M Bis-Tris-Propane, pH 4.1, 16% PEG 3,350; Cryoprotection in 25% PEG 3,350 |
