6D07
Crystal structure of the complex between human chromobox homolog 1 (CBX1) and H3K9me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-29 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.016, 32.124, 34.053 |
| Unit cell angles | 99.05, 106.20, 102.42 |
Refinement procedure
| Resolution | 26.336 - 2.100 |
| R-factor | 0.2094 |
| Rwork | 0.204 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fdt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.515 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.340 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.073 | 0.208 |
| Number of reflections | 6737 | |
| <I/σ(I)> | 8.5 | 2.6 |
| Completeness [%] | 93.5 | 86.7 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2 M sodium chloride, 0.1 M Tris, pH 8.0, 30% w/v PEG3350 |
