6CZX
Crystal structure of Arabidopsis thaliana phosphoserine aminotransferase isoform 1 (AtPSAT1) in complex with PLP internal aldimine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.221, 105.929, 186.886 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.780 - 1.570 |
R-factor | 0.15755 |
Rwork | 0.157 |
R-free | 0.17524 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xk1 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.541 |
Data reduction software | XDS (May 1, 2016) |
Data scaling software | XDS (May 1, 2016) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.780 | 1.660 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.089 | 0.965 |
Number of reflections | 230909 | 36591 |
<I/σ(I)> | 13.33 | 1.85 |
Completeness [%] | 99.2 | 98.1 |
Redundancy | 7.4 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2 M lithium sulfate, 17% PEG 3350 and 0.1 M Tris at pH 8.5 |