6CZU

BRD4(BD1) complexed with 3219

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	43.066, 48.967, 59.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.340 - 1.470
R-factor	0.1474
Rwork	0.145
R-free	0.19420
RMSD bond length	0.023
RMSD bond angle	2.246
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.520
High resolution limit [Å]	1.470	3.170	1.470
Rmerge	0.129	0.109	0.483
Total number of observations	148794
Number of reflections	22125	2362	2168
<I/σ(I)>	17
Completeness [%]	99.9	99	100
Redundancy	6.7	6.5	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	297	0.2 M Ammonium Sulfate, 0.1 M Bis-Tris pH 6.5, 25% PEG 3350