6CZQ
A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | BRUKER METALJET |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-04 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.3148 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.273, 66.726, 64.676 |
Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
Resolution | 26.866 - 2.200 |
R-factor | 0.2007 |
Rwork | 0.198 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.855 |
Data reduction software | XPREP |
Data scaling software | APEX 2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.866 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 16535 | 1618 |
<I/σ(I)> | 14.44 | |
Completeness [%] | 99.8 | 99.81 |
Redundancy | 6.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 0.1 M Sodium citrate tribasic dihydrate 0.5 M Ammonium sulfate 1.5 M Lithium sulfate monohydrate |